Recombination of Hybrid Target Sites by Binary Combinations of Flp Variants: Mutations that Foster Interprotomer Collaboration and Enlarge Substrate Tolerance

Jay H Konieczka; Andrew Paek; Makkuni Jayaram; Yuri Voziyanov; Yuri Voziyanov

doi:10.1016/j.jmb.2004.03.060

Recombination of Hybrid Target Sites by Binary Combinations of Flp Variants: Mutations that Foster Interprotomer Collaboration and Enlarge Substrate Tolerance

Jay H Konieczka
, Andrew Paek
, Makkuni Jayaram
, Yuri Voziyanov
, Yuri Voziyanov

Biological Sciences

University of Texas at Austin

Research output: Contribution to journal › Article › peer-review

Original language	American English
Journal	Journal of Molecular Biology
Volume	339
Issue number	2
DOIs	https://doi.org/10.1016/j.jmb.2004.03.060
State	Published - May 1 2004

Disciplines

Genetics
Biology
Molecular Biology

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10.1016/j.jmb.2004.03.060License: Unspecified

Cite this

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title = "Recombination of Hybrid Target Sites by Binary Combinations of Flp Variants: Mutations that Foster Interprotomer Collaboration and Enlarge Substrate Tolerance",

author = "Konieczka, \{Jay H\} and Andrew Paek and Makkuni Jayaram and Yuri Voziyanov and Yuri Voziyanov",

note = "JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page. Strategies of directed evolution and combinatorial mutagenesis applied to the Flp site-specific recombinase have yielded recombination systems that utilize bi-specific hybrid target sites. A hybrid site is assembled from two half-sites, each harboring a distinct binding specificity.",

year = "2004",

month = may,

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doi = "10.1016/j.jmb.2004.03.060",

language = "American English",

volume = "339",

journal = "Journal of Molecular Biology",

number = "2",

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T1 - Recombination of Hybrid Target Sites by Binary Combinations of Flp Variants: Mutations that Foster Interprotomer Collaboration and Enlarge Substrate Tolerance

AU - Konieczka, Jay H

AU - Paek, Andrew

AU - Jayaram, Makkuni

AU - Voziyanov, Yuri

N1 - JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page. Strategies of directed evolution and combinatorial mutagenesis applied to the Flp site-specific recombinase have yielded recombination systems that utilize bi-specific hybrid target sites. A hybrid site is assembled from two half-sites, each harboring a distinct binding specificity.

PY - 2004/5/1

Y1 - 2004/5/1

UR - https://www.sciencedirect.com/science/article/pii/S0022283604003754

U2 - 10.1016/j.jmb.2004.03.060

DO - 10.1016/j.jmb.2004.03.060

M3 - Article

VL - 339

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 2

ER -

Recombination of Hybrid Target Sites by Binary Combinations of Flp Variants: Mutations that Foster Interprotomer Collaboration and Enlarge Substrate Tolerance

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